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Research and Training at the MMM College of Health Sciences

Department of Genetics

List of Experiments

 

1

Isolation of Genomic DNA from Bacteria , Plant & Animal cells

2

Isolation of Plasmid DNA

3

RNA Isolation (Plant & Animal cells)

4

Agarose Gel Electrophoresis

5

Southern blotting, Western Blotting Northern blotting & Substractive hybridization

6

Restriction Digestion & Ligation

7

DNA Electro elution & Dialysis

8

Restriction Fragment Length Polymorphism- RFLP

9

Polymerase chain Reaction - PCR

10

Reverse Transcriptase PCR & RAPD PCR

11

Competent cell preparation & Bacterial Transformation

12

Bacterial Transduction

13

Bacterial Conjugation

14

Lamda phage DNA Isolation & Phage Titration

15

Mutagenesis - UV & Chemical

16

Isolation of Genomic DNA from Danio rerio skeletal Muscle

17

Isolation of mRNA from Danio rerio cardiomyocytes

18

Restriction Fragment Length Polymorphism of Danio rerio genome

19

Cell culture of Danio rerio cardiomyocytes & Induction of hypoxia and apoptosis

20

Differential staining and fluorescent microscopy

21

Culture of Human Leukocytes

22

Cell fusion - Leukocytes and Pituitary cells

23

Karyotyping

24

Chromatographic techniques

25

Isolation and fractionation of plant compounds

26

Separation of proteins using affinity beads

27

Isolation of cellular proteins by salting out and dialysis

28

SDS PAGE & Silver staining

29

Separation of proteins and mRNA on SDS PAGE

30

Immobilization of enzymes and study of enzyme kinetics

31

Design of microparticles for drug delivery

32

Identification of IAP protein domains

33

Design of peptide aptamers

34

Immuno precipitation, Immunoelecrophoresis , Immunodiffusion

35

Growth curve of Bacteria

36

Antibiotic sensitivity test

37

Protoplast Isolation and purification

38

Culture of HeLaS3 cells

39

Preparation of chicken embryo fibroblast

40

Study of apoptosis and in situ detection of apoptotic cells

 
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